As a practical option for transportation across the Isthmus, the railroad is not really an option. The only passenger service offered is M-F, 7AM Panama to Colon and a return trip to Panama City a little after 5PM. Just one round trip a day. I have been gone too long to knowledgeably comment on bus travel, but I can assure you unless you are well versed in third world travel, that is not a real good option either.